Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anusuya Ramasubramanian
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:31
HlyA Signal Sequence (Freiburg-compatible)
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K223054_sequence (Version 1) |
Description
The Hemolysin A signal sequence is generally fused with a second protein. Together with the Hly complex (HlyR, HlyC, HlyB and HlyD) and TolC, HlyAs can be used to take large proteins out of the cell.Notes
This part is Freiburg compatibleSource
Compilation of several papers:1. Cloning and hemolysin-mediated secretory expression of a codon-optimized synthetic human interleukin-6 gene in Escherichia coli
Yuanyi Li, Carol X. Chen1, Bernd-Ulrich von Specht and Heinz P. Hahn
Protein Expression and Purification 25 (2002) 437???447
2. Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins
J. Hess, I.Gentschev, W. Goebel and T. Jarchau
Mol Gen Genet (1990) 224:201-208
3. Development of antigen-delivery systems, based on the Escherichia coli hemolysin secretion pathway
Ivaylo Gentschev, Hans Mollenkopf , Zeljka Sokolovic, Jorgen Hess, et al.
Gene. 1996 Nov 7;179(1):133-40.
4. Mutations affecting activity and transport of haemolysin in Escherichia coli
Albrecht Ludwig, Monika Vogel and Werner Goebel
Mol Gen Genet. 1987 Feb;206(2):238-45.