Types | DnaRegion
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Roles | CDS
Coding
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Sequences | BBa_K227005_sequence (Version 1)
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Description
puc B is the LHII beta transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. PucB forms a functional LHII complex only with pucA, and data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.
Notes
puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A functional LHII complex requires both puc A and B. Data suggests that A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.
Source
PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.