BBa_K227005

BBa_K227005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K227005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WashU iGEM 2009
Date created: 2009-10-11 11:00:00
Date modified: 2015-05-08 01:11:32

puc B coding region from R. sphaeroides



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K227005_sequence (Version 1)

Description

puc B is the LHII beta transmembrane protein coding region found naturally in Rhodobacter Sphaeroides. LHII complexes form the light-harvesting antennae the funnel energy down to LHI and the reaction center. They are naturally found in the ratio of 3-6.7 LHII per LHI. PucB forms a functional LHII complex only with pucA, and data suggests the precence of puc C,D, and E regions also contribute to the formation of a functional protein.

Notes

puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A functional LHII complex requires both puc A and B. Data suggests that A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.

Source

PCR applified from plasmid pRKCBC3 provided by Dr. Neil Hunter.

Sequence Annotation Location Component / Role(s)
pucB
1,156
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K227005/1