BBa_K228000

BBa_K228000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K228000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lin Min

Date created: 2009-08-24 11:00:00

Date modified: 2015-05-08 01:11:32

T7ptag(T7polymerase with amber mutation)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K228000_sequence (Version 1)

Description

T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor [[BBa_K228001]] SupD-tRNA, it can be translated into functional T7 polymerase.

T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)

Source

The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,2673	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K228000/1

Member of these Collections

• iGEM Parts Registry