Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Min
Date created: 2009-08-24 11:00:00
Date modified: 2015-05-08 01:11:32
T7ptag(T7polymerase with amber mutation)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K228000_sequence (Version 1) |
Description
T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor [[BBa_K228001]] SupD-tRNA, it can be translated into functional T7 polymerase.T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)