Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:35
YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K233306_sequence (Version 1) |
Description
The Tat (twin-arginine translocation) system of Escherichia coli serves to translocate folded proteins across the cytoplasmic membrane. The reasons established so far for the Tat dependence are cytoplasmic cofactor assembly and/or heterodimerization of the respective proteins. We were interested in the reasons for the Tat dependence of novel Tat substrates and focused on two uncharacterized proteins, YcdO and YcdB. Both proteins contain predicted Tat signal sequences. However, we found that only YcdB was indeed Tat-dependently translocated, whereas YcdO was equally well translocated in a Tat-deficient strain. YcdB is a dimeric protein and contains a heme cofactor that was identified to be a high-spin Fe(III)-protoporphyrin IX complex. In contrast to all other periplasmic hemoproteins analyzed so far, heme was assembled into YcdB in the cytoplasm, suggesting that heme assembly could take place prior to translocation. The function of YcdB in the periplasm may be related to a detoxification reaction under specific conditions because YcdB had peroxidase activity at acidic pH, which coincides well with the known acid-induced expression of the geneNotes
We had to add a base at the end of the tag in such a way that when it was fused with the gene of the protein to be secreted, it would be an in-frame after the scar that would result due to standard assembly.we therefore chose to add a 'C' to the end of the designed export tag to allow it to be fused in frame. this caused an addition of alanine when translated.
we checked the changes in secondary structure that this addition caused by the available online tools like SOPMA and SSpro.
no significant changes were predicted