Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marc T. K. Nielsen
Date created: 2009-08-02 11:00:00
Date modified: 2015-05-08 01:11:36
RIP +export signal (OmpA). Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K237003_sequence (Version 1) |
Description
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth.RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice.
References will come later.
This is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's.
We can at this point in our project not verify that RIP will be exported by our Coli's.
We have come to affectionately call this brick sRIP (short for signal-RIP, in contrast to just RIP).
Notes
This sequence is reverse-engineered from the amino-acid sequence by using the commonly used codons of E. Coli.It is designed to provide a big output of peptide. We just hope that this output won't kill the cell.