BLp

BBa_K238013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K238013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Annelien Verfaillie

Date created: 2009-08-01 11:00:00

Date modified: 2015-05-08 01:11:36

Exact promoter of the blue light receptor

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K238013_sequence (Version 1)

Description

ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)

Notes

none

Source

E.coli strain MC4100

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35 box	28,33	promoter, feature/promoter
-10 box	52,58	feature/promoter, promoter
spacer	34,51	sequence_feature, feature/misc
inverted repeat part 1	53,59	sequence_feature, feature/misc
inverted repeat part 2	67,73	feature/misc, sequence_feature
transcription start	64,65	feature/start, start_codon

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K238013/1

Member of these Collections

• iGEM Parts Registry