BBa_K239000

BBa_K239000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-19 11:00:00

Date modified: 2015-05-08 01:11:36

DegP promoter, activated by Phosporylated CpxR and SigmaE

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K239000_sequence (Version 1)

Description

Sequence contains 3 phosphorylated CpxR binding sites and transcription is initiated by sigma factor E (24). A native sequence has been used but it has been modified to remove Spe1 restriction site.
Function: Can detect various periplasmic stresses (DegP is expressed by the cell to repair damage in the periplasm).
Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.

Notes

Detect and remove restriction sites: Xba1, EcoRI, Pst1, Spe1.
Some uncertanty about where to start and stop in the sequece. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CpxR	4,19	non_covalent_binding_site, feature/binding
CpxR	154,171	non_covalent_binding_site, feature/binding
CpxR	164,178	non_covalent_binding_site, feature/binding
-35 box	201,206	promoter, feature/promoter
-10 box	223,227	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239000/1

Member of these Collections

• iGEM Parts Registry