BBa_K242201

BBa_K242201 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K242201
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jes??s Abraham Avelar Rivas
Date created: 2009-10-10 11:00:00
Date modified: 2015-05-08 01:11:37

Colicin E9 DNase domain



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K242201_sequence (Version 1)

    Description

    Colicin E9 is able to degrade DNA. This DNase domain extracted by us is enough to get this function. It is inhibited in the presence of its immunity protein 9.

    Notes

    We took the sequence designed by Russell WALLIS et al. We included a RBS, eliminated preffix/suffix restriction sites and added two stop codons (ATT).

    Source

    Escherichia coli strain J plasmid ColE9-J

    Sequence Annotation Location Component / Role(s)
    rbs
    start
    E9 DNase Domain
    stop
    		1,12
    21,23
    21,425
    426,431
    		ribosome_entry_site feature/rbs
    start_codon feature/start
    CDS feature/cds
    feature/stop stop_codon

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K242201/1
     

    Attachments

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