Fok_a

BBa_K243000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K243000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:37

Protein domain (active) of the restriction endonuclease FokI



    • Details

    Types
    DnaRegion

    Roles
    Protein_Domain

    polypeptide_domain

    Sequences BBa_K243000_sequence (Version 1)

    Description

    This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).

    Notes

    Modifications of the vector (catalytic active heterodimer)
    -heterodimeric aminio acids

    * switch Glutamate 490/310-312 to Lysin (GAA->AAA)
    * switch isoleucin 538/454-456 to Lysin (ATC->AAA)

    Source

    extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene

    Sequence Annotation Location Component / Role(s)
    Fok_a
    		1,579
    		CDS feature/protein

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K243000/1
     

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