Fok_a

BBa_K243000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K243000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:37

Protein domain (active) of the restriction endonuclease FokI



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K243000_sequence (Version 1)

Description

This part is used as the active domain of our universal restriction endonuclease. It cut double stranded DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001).

Notes

Modifications of the vector (catalytic active heterodimer)
-heterodimeric aminio acids

* switch Glutamate 490/310-312 to Lysin (GAA->AAA)
* switch isoleucin 538/454-456 to Lysin (ATC->AAA)

Source

extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene

Sequence Annotation Location Component / Role(s)
Fok_a
1,579
CDS feature/protein
igem#experience Works  
igem#sampleStatus It's complicated  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K243000/1