Fok_i

BBa_K243001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K243001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Freiburg Bioware09

Date created: 2009-10-07 11:00:00

Date modified: 2015-05-08 01:11:37

Protein domain (inactive) of the restriction endonuclease FokI

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K243001_sequence (Version 1)

Description

This part is used as the inactive domain of our universal restriction endonuclease. It fused with the active protein domain of our universal restriction endonuclease(BBa_K243000)and linked with specific oligos.

Notes

Modifications of the vector (catalytic inactive heterodimer)
-heterodimeric amino acids

* switch Glutamin 486/298-300 to Glutamate (CAA->GAA)
* switch Isoleucin 499/337-339 to Leucin (ATC->CTG)

-catalytic amino acids

* switch Aspartate 450/190-192 to Alanin (GAC->GCG)
* switch Aspartate 467/243-245 to Alanin (GAT->GCG)

Source

extract coding region of Fok from the restriction-modification genes of the chromosomal DNA of Flavobacterium okeanokoites. Part synthesized by Mr.Gene

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Fok_i	1,579	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K243001/1

Member of these Collections

• //cds/enzyme/endonuclease/restriction
• iGEM Parts Registry