DsbA

BBa_K243002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K243002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:37

DsbA signal sequence (enables periplasm export)



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K243002_sequence (Version 1)

Description

The signal sequence of DsbA linked to an cds of e.g.a protein, applies the exportation of the protein to the periplasm. The accumulation of the expressed protein in the periplasm, can be used for the purification of the protein.

Notes

the signal sequence had to be located at the N-terminus of the (fusion)protein.

Source

Sequence of the DsbA was copied out from E.coli B genom
Synthesized oligos with signal sequence of DsbA by sigma.

Sequence Annotation Location Component / Role(s)
Dsba signal sequence
1,54
tag feature/tag
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K243002/1