DigA

BBa_K243003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K243003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Freiburg Bioware09

Date created: 2009-10-11 11:00:00

Date modified: 2015-05-08 01:11:37

Digoxigenin binding protein (DigA)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K243003_sequence (Version 1)

Description

Digoxigenin is a steroid extracted from the plant Digitalis purpurea and D.lanata. Digoxigenin modified
oligonucleotides are widely used as high sensitivity probes for non-radioactive immunoassay s and hybridization
experiments. These DIG-probes are monitored by anti-DIG antibodies. These antibodies only show cross-reactivity with blossoms and leaves of D. spec(sole natural sources for DIG) ??? so they are suitable for analysis of any other biological species. Anti-DIG antibodies are either modified with fluorescent dyes (direct detection) or with an enzyme (indirect detection via enzyme-substrate reaction).

Notes

none

Source

synthesized by purimex

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Digoxigenin tag	1,552	tag, feature/tag

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K243003/1

Member of these Collections

• //cds/receptor/antibody
• iGEM Parts Registry