Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cihan Tastan
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:42
Vitreoscilla hemoglobin (VHb) protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K258014_sequence (Version 1) |
Description
The gene coding for the Vitreoscilla hemoglobin (VHb) molecule has been cloned and functionally expressed in Escherichia coli. By using a plasmid-encoded gene as well as single-copy integrants, the oxygen-dependent VHb gene (VHb) promoter was shown to be functional in E. coli. The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation).We wanted to measure the activity of VHb gene; therefore, the VHb promoter. The VHb promoter becomes active when the surrounding oxygen amount lowers to 2%. We wanted to see whether this promoter will effectively work with our E. Coli cells.