Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-07-13 11:00:00
Date modified: 2015-05-08 01:11:42
fiu ??? Outer Membrane Ferric Iron Transporter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K259001_sequence (Version 1) |
Description
Biology of Fiu
The fiu gene encodes for an outer membrane transporter found in E.coli, a gram negative bacterium. It is involved in the active transport (energy dependent uptake) of ferric iron from the extracellular environment. This outer membrane receptor can only bind ferric iron and is a catecholate siderophore. For uptake into the cell to be completed fiu has to complex with another protein known as TonB (a transperiplasmic protein) that will carry out the active transport by using ATP as an energy source.
What this Biobrick gives you upon expression
This biobrick encodes ONLY for fiu.
Fiu Statistics
Fiu belongs to the family of Ton-B dependent receptors and is related to fhuA, hence it also has a beta-barrel structure.
{| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2"
!Chassis
!Monomer/Multimer
!Aminoacid Length/Weight
!Localisation
!Structure
|-
|E.Coli
|Monomer
|760/81.96kDa
|Outer Membrane/OMV
|beta-barrel
|}
References
E.Coli wiki net
Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
Ecogene number EG13317
UniProt Accession number P75780
Notes
In order to comply with assembly standard 10 (RFC10) the stop codon for fiu had to be changed to TAA.Source
Fiu gene is part of the chromosomal E.coli genome. In particular it is found at 18.07min with orientation in the counter-clockwise direction.Further details at EcoGene
| Sequence Annotation | Location | Component / Role(s) |
| start stop Signal Sequence Multipass protein (OM-Periplasm) | 1,3 2243,2247 1,99 100,2247 | start_codon feature/start stop_codon feature/stop feature/misc sequence_feature feature/misc sequence_feature |