Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:42
pCC2FosMB
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K260001_sequence (Version 1) |
Description
This fosmid backbone was generated by recombineering-mediated transfer of the HincII fragment from BBa_K260016 (inside backbone BBa_K260010) to pCC2FosM (BBa_K260000). This reaction also transferred the blasticidin resistance gene (BsdR), which is now part of this backbone. pCC2FosMB is available with part BBa_K260016 between prefix and suffix.Notes
The insertion site for BsdR was chosen to be upstream of MECP2 exon 1 but still with more than 10 kb of MECP genomic sequence upstream.Source
Based on BBa_K260000, so it also contains human MECP2 genomic sequence, but has an insert taken from BBa_K260010.| Sequence Annotation | Location | Component / Role(s) |
| suffix prefix CmR SpecR BsdR gb3 promoter | 1,21 48151,48172 22815,23474 18108,18782 116,514 22,115 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/cds CDS CDS feature/cds CDS feature/cds promoter feature/promoter |