Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:42
pRhaFlp. [L-rhamnose] -> FLP(F70L)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K260003_sequence (Version 1) |
Description
This plasmid is based on pSC101 and contains the rhamnose operon (rhaR and rhaS genes) as well as the F70L variant of FLP recombinase under rhamnose-promoter control. Therefore L-rhamnose can be used to induce FLP(F70L) expression.The pSC101 origin of replication is temperature sensitive due to a mutation of repA. Permisive temperature is 30 degC. 37 degC should be avoided as the plasmid can not replicate.
Notes
This plasmid comes from Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods. 2006 Oct;3(10):839-44. However, it was modified by deletion of dhfr gene and RedABGRecA operon through insertion of a kanamycin resistance cassette.Source
dhfr had to be deleted as the same gene for trimpethoprim resistance was going to be used elsewhere in the same cell.| Sequence Annotation | Location | Component / Role(s) |
| suffix repA pSC101 origin KanR T4_32 terminator FLP(F70L) rhaS rhaR T4_32 terminator rhamnose-promoter prefix | 1,21 108,1058 1442,1803 1854,2648 2902,2933 2954,4225 4513,5349 5333,6271 6272,6303 4226,4512 6324,6345 | engineered_region feature/BioBrick feature/cds CDS feature/misc sequence_feature CDS feature/cds stem_loop feature/stem_loop feature/cds CDS CDS feature/cds feature/cds CDS stem_loop feature/stem_loop feature/promoter promoter feature/BioBrick engineered_region |