Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42
pMA-@CC2FosMB-00500bp
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K260004_sequence (Version 1) |
Description
This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 00500 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.The pMA-@CC2FosMB-00500bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260004|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 00500 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 500 bp exactly.
These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:
[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp
Notes
The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260004|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].Source
Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].| Sequence Annotation | Location | Component / Role(s) |
| suffix BBa_K260001 (pCC2FosMB) 3' homology FRT-00500bp PvuII site PvuII site AmpR ColE1 origin BBa_K260001 (pCC2FosMB) 5' homology FRT-00500bp prefix | 1,21 22,91 92,97 2468,2473 609,1469 1617,2284 2474,2543 2544,2565 | feature/BioBrick engineered_region sequence_feature feature/misc sequence_feature feature/dna sequence_feature feature/dna CDS feature/cds sequence_feature feature/misc feature/misc sequence_feature feature/BioBrick engineered_region |