BBa_K260006

BBa_K260006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K260006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42

pMA-@CC2FosMB-02000bp



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K260006_sequence (Version 1)

Description

This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 02000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.

The pMA-@CC2FosMB-02000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260006|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 02000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 2,000 bp exactly.

These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:

[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp

Notes

The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260006|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].

Source

Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].

Sequence Annotation Location Component / Role(s)
suffix
BBa_K260001 (pCC2FosMB) 3' homology FRT-02000bp
PvuII site
PvuII site
AmpR
ColE1 origin
BBa_K260001 (pCC2FosMB) 5' homology FRT-02000bp
prefix
1,21
22,91
2468,2473
92,97
609,1469
1617,2284
2474,2543
2544,2565
feature/BioBrick engineered_region
feature/misc sequence_feature
feature/dna sequence_feature
feature/dna sequence_feature
CDS feature/cds
feature/misc sequence_feature
sequence_feature feature/misc
feature/BioBrick engineered_region
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K260006/1