BBa_K260007

BBa_K260007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K260007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42

pMA-@CC2FosMB-05000bp



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K260007_sequence (Version 1)

Description

This plasmid backbone is based on pMA from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 05000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.

The pMA-@CC2FosMB-05000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260007|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 05000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 5,000 bp exactly.

These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:

[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp

Notes

The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260007|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].

Source

Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].

Sequence Annotation Location Component / Role(s)
suffix
BBa_K260001 (pCC2FosMB) 3' homology FRT-05000bp
PvuII site
ColE1 origin
PvuII site
AmpR
BBa_K260001 (pCC2FosMB) 5' homology FRT-05000bp
prefix
1,21
22,91
92,97
1617,2284
2468,2473
609,1469
2474,2543
2544,2565
engineered_region feature/BioBrick
feature/misc sequence_feature
feature/dna sequence_feature
sequence_feature feature/misc
feature/dna sequence_feature
feature/cds CDS
feature/misc sequence_feature
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K260007/1