BBa_K260008

BBa_K260008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K260008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaj Bernhardt
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:42

pMA-RQ-@CC2FosMB-10000bp



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_K260008_sequence (Version 1)

Description

This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 50 bp of homology arms for insertion of the contained BioBrick into the 10000 locus of pCC2FosMB ([[Part:BBa_K260001|]]) by recombineering.

The pMA-RQ-@CC2FosMB-10000bp backbone is available with [[Part:BBa_K260015|]], which is composed of promoter-FRT-dhfr. If the PvuII fragment from [[Part:BBa_K260008|]]-[[Part:BBa_K260015|]] is transferred by recombineering to the the 10000 locus of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]], the intervening sequence between the FRT sites of [[Part:BBa_K260015|]] and [[Part:BBa_K260016|]] on the [[Part:BBa_K260001|]] backbone will be 10,000 bp exactly.

These plasmid backbones are similar and all allow recombineering-mediated transfer of the contained BioBrick (typically [[Part:BBa_K260015|]]) to the pCC2FosMB backbone [[Part:BBa_K2600001|]]. They differ in the homology arms, which determines the locus of insertion into pCC2FosMB. The bp numbers denote final inter-FRT distance as described above:

[[Part:BBa_K260004|]]: pMA-CC2FosMB-00500bp
[[Part:BBa_K260005|]]: pMA-CC2FosMB-01000bp
[[Part:BBa_K260006|]]: pMA-CC2FosMB-02000bp
[[Part:BBa_K260007|]]: pMA-CC2FosMB-05000bp
[[Part:BBa_K260008|]]: pMA-RQ-CC2FosMB-10000bp

Notes

The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260008|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].

Source

Geneart plasmid pMA-RQ, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].

Sequence Annotation Location Component / Role(s)
suffix
BBa_K260001 (pCC2FosMB) 3' homology FRT-10000bp
PvuII site
PvuII site
AmpR
ColE1 origin
BBa_K260001 (pCC2FosMB) 5' homology FRT-10000bp
prefix
1,21
22,91
92,97
2451,2456
1087,1947
272,939
2457,2526
2527,2548
engineered_region feature/BioBrick
sequence_feature feature/misc
sequence_feature feature/dna
sequence_feature feature/dna
feature/cds CDS
feature/misc sequence_feature
sequence_feature feature/misc
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K260008/1