BBa_K268001

BBa_K268001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K268001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:43

pRS316



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K268001_sequence (Version 1)

Description

Empty plasmid for gene expression in S. cerevisiae. Ura selection in yeast and ampicillan resistance in E. coli. One of a series of pBluescript-based centromere vectors (ATCC 77142 ATCC 77145, ATCC 77157 to ATCC 77158) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4.

Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.8; PvuII--4.2, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416309 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS313, pRS315, pRS316.

Notes

Eliminated PstI, XbaI and SpeI restriction sites.

Source

From ATCC. Pubmed accession number U03442.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K268001/1