Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:43
YGL219W -> GFP
| Types | DnaRegion |
| Roles | primer Primer |
| Sequences | BBa_K268023_sequence (Version 1) |
Description
~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YGL219W gene. For transformation in S. cerevisiae only, this part will swap out the YGL219 gene and replace it with a Kan marker+GFP coding region downstream of the YGL219W promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers:
forward: 64-mer CACAATTGAGTACTAAAAGCTTTCGTATCTTACCCAATATCATGcggatccccgggttaattaa
reverse: 58-mer GTTCATGTATTTTTTTCCAGTTACTAAAAACCGGAAGAgaattcgagctcgtttaaac
Source
Promoter region from gene YGR035C in S. cerevisiae as obtained through yeastgenome.org.Gene deletion and tagging cassette from Longtine, et al (1998).