Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:44
YHL012W -> GFP
| Types | DnaRegion |
| Roles | primer Primer |
| Sequences | BBa_K268026_sequence (Version 1) |
Description
~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YHL012W gene. For transformation in S. cerevisiae only, this part will swap out the YHL012 gene and replace it with a Kan marker+GFP coding region downstream of the YHL012W promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers:
Forward: 63-mer GAAAAATATTTCACTGTTCTACATAGTATATACAAGATCAATGcggatccccgggttaattaa
Reverse: 62-mer GAAAACTTTATTCATGCATTTTACCCAAAAGTTTCTCTTGTgaattcgagctcgtttaaac
Source
Promoter region from gene YHL012W in S. cerevisiae as obtained through yeastgenome.org.Gene deletion and tagging cassette from Longtine, et al (1998).