BBa_K268027

BBa_K268027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K268027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nora Yucel
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:44

YDL205C -> GFP



Types
DnaRegion

Roles
Primer

primer

Sequences BBa_K268027_sequence (Version 1)

Description

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YDL205C gene. For transformation in S. cerevisiae only, this part will swap out the YDL205 gene and replace it with a Kan marker+GFP coding region downstream of the YDL205C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers:
Foward: 62-mer CACAGAAAAAAAGAAAATATAATAAATTAGTATTGCGATATGcggatccccgggttaattaa
Reverse: 60-mer CGCAAAACAGAGGGTTCGAAGGAAAACAGGAAACCTCTACgaattcgagctcgtttaaac

Source

Promoter region from gene YDL205C in S. cerevisiae as obtained through yeastgenome.org.
Gene deletion and tagging cassette from Longtine, et al (1998).

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K268027/1