Types | DnaRegion
|
Roles | engineered_region
Reporter
|
Sequences | BBa_K271000_sequence (Version 1)
|
Description
Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-purple color, aryl acylamidase gene will apply to reporter gene.
Notes
ATGGGTAAGTCACATTCGCCAGTTCACTGGAAGTCGGCGGCCGAGATCGTCGAGTTAGTCAAGAGCAAACAAATTTCCCC
ACGCGAGGTCGTGGAAAGCACGATCGACCTCATCGAGCAACGCGATCCCGGCCTGAATGCGGTCGTGTACAAGGCTTACG
ATGAAGCGCGCGAAAAAGCGGCTGCGCTTGAGCGGCGCATCATGCAAGGCGAGCCTGTTGGCATGCTGGCGGGGGTTCCG
ACTCTCATGAAGGACTTGTTCGCGGCCAAGCCGGGCTGGCCGTCCACGCTGGGCGGAATTCGCGCCCTCAAGGACGCCCG
CGGCGCTGCCGGCGTCTGGTCCACTTATCCATTGAAGATGTCCGGCGAAGACAGCCTGTTGCTGGGACAGACAAACAGCC
CCGTCTATGGCTTTCGCGGAACCACGGACAACACGTTTTTCGGTCCGACGCGCAATCCATTCAACCTCGACTTCAACGCA
GGGGGGTCTTCGGGAGGAGCCGCTGCGCTCGTGGCCGATGGCATCGTTCCTGTGGCAGGCGGCACGGATGGCGGCGGCTC
GATCCGCATTCCGGCGGCATGGACCAATACCTACGGCTTTCAGCCTTCGATAGGACGCGTTCCGTTCAAAAGCCCGCCCC
AATGCTTTCCATCCGGGGACCGTATCTCTATGAAGGAGCCCATCACGCGGACGGTGCCGGATGCGGCATTGGCAATGAAT
GTACTGCACGGCTTCGACCGCCGCGATCCTGCTTCGCTGCGAGTCAAGCTGGACTTCACCTCAGCGCTTGCACAAGGCGT
GCGTGGCAAAAAAATCGGCCTGACGCTGAATTACGGGGTATTTCCCGTCCAGCAAGAGATTCAGGATTTGATCGGCAAGG
CTGCCCGGGTTTTTACAGAGCTTGGCGCACACGTCGAATTCGTCGACCTCGGTATCCCCTACAGCCAGAAACAAATGAGC
GATGCCTGGTGCCGCATGATCGCGATTCCTACTGTCGCCTCGATGCAGGCGCTGCGCAAGGAAGGCATTGACTTATATGG
CGAACATCGGGCAGACATACCCGATGCGCTGATGAAGTGGATAGATGCGGTCGCTGACATAAGCGTCCAGCAGATCAGCG
CTGATCAACTATTGAGAACGACCGTTTTCGATTGCATGAACGGCGTATTCGATCGCTTTGACCTATTATTGGCGCCGACC
CTGGCATGCATGCCTGTTCGCAATGCGACTGATGGCTGCACCGAGGGACCAAGCCAGATCAACGGTGAAGAAATCGACCC
TTTGATCGGCTGGTGCATGACTTACCTGACCAATTTTTCAGGGCATCCCAGTGCCAGTGTCCCGGCTGGCCTGATTGATG
GCCTGCCGGCAGGCATGCTCATTATCGGCGATCGCCAGGCGGACCTGGATGTCATTGCCGCAAGCGCCGCTTTCGAGCGA
GCCAGCCCGTGGTCCCAATACTATGACATTCCCGCCGGACGGCCCCTGTGATAA
Source
soil bacterium