BBa_K271000

BBa_K271000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K271000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanseong Roh
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:44

Aryl acylamidase



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K271000_sequence (Version 1)

Description

Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-purple color, aryl acylamidase gene will apply to reporter gene.

Notes

ATGGGTAAGTCACATTCGCCAGTTCACTGGAAGTCGGCGGCCGAGATCGTCGAGTTAGTCAAGAGCAAACAAATTTCCCC
ACGCGAGGTCGTGGAAAGCACGATCGACCTCATCGAGCAACGCGATCCCGGCCTGAATGCGGTCGTGTACAAGGCTTACG
ATGAAGCGCGCGAAAAAGCGGCTGCGCTTGAGCGGCGCATCATGCAAGGCGAGCCTGTTGGCATGCTGGCGGGGGTTCCG
ACTCTCATGAAGGACTTGTTCGCGGCCAAGCCGGGCTGGCCGTCCACGCTGGGCGGAATTCGCGCCCTCAAGGACGCCCG
CGGCGCTGCCGGCGTCTGGTCCACTTATCCATTGAAGATGTCCGGCGAAGACAGCCTGTTGCTGGGACAGACAAACAGCC
CCGTCTATGGCTTTCGCGGAACCACGGACAACACGTTTTTCGGTCCGACGCGCAATCCATTCAACCTCGACTTCAACGCA
GGGGGGTCTTCGGGAGGAGCCGCTGCGCTCGTGGCCGATGGCATCGTTCCTGTGGCAGGCGGCACGGATGGCGGCGGCTC
GATCCGCATTCCGGCGGCATGGACCAATACCTACGGCTTTCAGCCTTCGATAGGACGCGTTCCGTTCAAAAGCCCGCCCC
AATGCTTTCCATCCGGGGACCGTATCTCTATGAAGGAGCCCATCACGCGGACGGTGCCGGATGCGGCATTGGCAATGAAT
GTACTGCACGGCTTCGACCGCCGCGATCCTGCTTCGCTGCGAGTCAAGCTGGACTTCACCTCAGCGCTTGCACAAGGCGT
GCGTGGCAAAAAAATCGGCCTGACGCTGAATTACGGGGTATTTCCCGTCCAGCAAGAGATTCAGGATTTGATCGGCAAGG
CTGCCCGGGTTTTTACAGAGCTTGGCGCACACGTCGAATTCGTCGACCTCGGTATCCCCTACAGCCAGAAACAAATGAGC
GATGCCTGGTGCCGCATGATCGCGATTCCTACTGTCGCCTCGATGCAGGCGCTGCGCAAGGAAGGCATTGACTTATATGG
CGAACATCGGGCAGACATACCCGATGCGCTGATGAAGTGGATAGATGCGGTCGCTGACATAAGCGTCCAGCAGATCAGCG
CTGATCAACTATTGAGAACGACCGTTTTCGATTGCATGAACGGCGTATTCGATCGCTTTGACCTATTATTGGCGCCGACC
CTGGCATGCATGCCTGTTCGCAATGCGACTGATGGCTGCACCGAGGGACCAAGCCAGATCAACGGTGAAGAAATCGACCC
TTTGATCGGCTGGTGCATGACTTACCTGACCAATTTTTCAGGGCATCCCAGTGCCAGTGTCCCGGCTGGCCTGATTGATG
GCCTGCCGGCAGGCATGCTCATTATCGGCGATCGCCAGGCGGACCTGGATGTCATTGCCGCAAGCGCCGCTTTCGAGCGA
GCCAGCCCGTGGTCCCAATACTATGACATTCCCGCCGGACGGCCCCTGTGATAA

Source

soil bacterium

Sequence Annotation Location Component / Role(s)
PyodA
Aryl acylamidase
double stop codon
AAV
1,271
272,1759
1793,1798
1760,1792
feature/promoter promoter
feature/protein CDS
stop_codon feature/stop
feature/tag tag
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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user/myers
 
synbiohub#topLevel
BBa_K271000/1