pdc

BBa_K273000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K273000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karl Brune
Date created: 2009-08-31 11:00:00
Date modified: 2015-05-08 01:11:44

Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K273000_sequence (Version 1)

Description

The pdc converts pyruvate to acetaldehyde. Acetaldehyde can be then furhter converted to ethanol via the adh2 (alcohol dehydrogenase).










Notes

none

Source

Source of the Pyruvate decarboxylase (pdc) is Z. Mobilis ATCC 29191 via Phusion PCR with the following primers:
pdc_forward CGCgaattcgcggccgcttctagATGAGTTATACTGTCGGTACC 44nt
pdc_reverse CGctgcagcggccgctactagtaTTATTAGAGGAGCTTGTTAACAGGC 48nt

Sequence Annotation Location Component / Role(s)
startcodon
stopcodon (double)
coding sequence
1,3
1705,1710
1,1704
feature/start start_codon
feature/stop stop_codon
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K273000/1