Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuna Gould
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:45
Vio operon ABDE
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K274003_sequence (Version 1) |
Description
Produces a green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioC gene removed by restriction with BamHI and BglII. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site.Notes
The origonal vio operon was designed with the vioC gene flanked by complementary sticky end restriction sites for BamHI and BglII. This allowed the vioC to be easily removed, leading to the production of green pigment.Source
This part was made from the vio operon biobrick, part number BBa_K274002. The vioC gene has been removed by restriction digest.| Sequence Annotation | Location | Component / Role(s) |
| rbs vioA misc vioB rbs vioD rbs vioE | 1,17 18,1274 1275,1291 1292,4294 4295,4312 4313,5439 5440,5456 5457,6032 | ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS |