Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Siming Ma
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:45
CrtY under Pbad promoter (I0500)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K274111_sequence (Version 1) |
Description
This construct is created by putting BBa_K118013 (rbs+CrtY) under the control of Pbad promoter (I0500), by standard assembly.CrtY (lycopene B-cyclase) converts lycopene (red) to beta-carotene (orange). When used in double-transformation together with a constitutive lycopene-producing device (e.g. BBa_K274110), the resulting system can produce colour change (red to orange) upon induction with arabinose.
Notes
The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). BBa_K2741x0 series (BBa_K274100, BBa_K274110 and BBa_K274120) contain enzyme cassette CrtEBI that convert farnesyl pyrophosphate to lycopene (red). BBa_K274111 (I0500+CrtY) then converts lycopene (red) to beta-carotene (orange).Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment production than strain TOP10.
Source
The (rbs+CrtY) component comes from BBa_K118013. The coding sequence for CrtY comes from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087).The Pbad promoter comes from BBa_I0500.
| Access | Instance | Definition |
| public public public | BBa_I0500 BBa_K118012 BBa_K118008 | pBad/araC BBa_K118012 BBa_K118008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_K118012 BBa_K118008 | 1,1210 1219,1222 1229,2380 | pBad/araC BBa_K118012 BBa_K118008 |