Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:45
3L.3_23.A1.07
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K277007_sequence (Version 1) |
Description
3L.3_23.A1.07 is 689 bases long and is cloned into the pGem-T vector.3L.3_23.A1.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.07 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
gene YCL069W (-788..588) Permease of basic amino acids in the vacuolar membrane
mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (220..231) removal of AlwNI
loxP_site loxPsym_YCL069W (592..625)
ARS ARS301 (686..+941) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer
Sequence (the first 689 bases correspond to coordinates 4461..5149 in synthetic chromosome yeast_chr3_3_23)