Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:45
3L.3_23.A2.06
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K277018_sequence (Version 1) |
Description
3L.3_23.A2.06 is 749 bases long and is cloned into the pGem-T vector.3L.3_23.A2.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.06 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
forward_primer YCL059C_tagf1v1 (191..218)
gene YCL059C (23..+973) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
reverse_primer YCL059C_tagr1v1 (641..665)
Sequence (the first 749 bases correspond to coordinates 12542..13290 in synthetic chromosome yeast_chr3_3_23)