BBa_K277031

BBa_K277031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K277031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:46

3L.3_23.B1.05



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K277031_sequence (Version 1)

Description

3L.3_23.B1.05 is 706 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.05 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
mutation_affecting_coding_sequence YCL054W_re_remove_MmeI (32..43) removal of MmeI
reverse_primer YCL054W_tagr2v1 (124..151)
gene YCL054W (-484..+2041) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants

Sequence (the first 706 bases correspond to coordinates 22137..22842 in synthetic chromosome yeast_chr3_3_23)

Notes

Constructed using overlap extension PCR protocol described in RFC38

Source

Synthetic Yeast

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K277031/1