Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:46
3L.3_23.B1.10
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K277036_sequence (Version 1) |
Description
3L.3_23.B1.10 is 678 bases long and is cloned into thepGem-T vector.
3L.3_23.B1.10 was designed as a piece of
synthetic chromosome 3 with the goal of minimizing and
stabilizing that chromosome and to that end has had any
tRNAs, introns, repeat regions, and transposons that were
present in the wildtype chromosome removed. In addition a
very few gene sequences were slightly recoded to add or
remove restriction enzyme recognition sites to facilitate
assembly; most gene sequences were slightly recoded to
introduce unique primers for diagnostic PCR
amplification, and some gene sequences were slightly
recoded to address the distribution of stop codon usage.
3L.3_23.B1.10 is a constituent of 3L.3_23.B1 (along with
3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03,
3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06,
3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09,
3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and
3L.3_23.B1.14.)
This part contains at least part of the
following features (positions offset from first base of
sequence):
kind and name offset notes
gene
YCL051W (217..+1968) Protein involved in control of
cell wall structure and stress response%3B inhibits Cbk1p
protein kinase activity%3B overproduction confers
resistance to cell-wall degrading enzymes
forward_primer YCL051W_tagf2v1 (630..657)
Sequence
(the first 678 bases correspond to coordinates
25852..26529 in synthetic chromosome yeast_chr3_3_23)