Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:46
3L.3_23.B3.07
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K277059_sequence (Version 1) |
Description
3L.3_23.B3.07 is 758 bases long and is cloned into the pGem-T vector.3L.3_23.B3.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B3.07 is a constituent of 3L.3_23.B3 (along with 3L.3_23.B3.01, 3L.3_23.B3.02, 3L.3_23.B3.03, 3L.3_23.B3.04, 3L.3_23.B3.05, 3L.3_23.B3.06, 3L.3_23.B3.08, and 3L.3_23.B3.09.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
gene YCL039W (-198..+2039) Protein of unknown function%2C involved in proteasome-dependent catabolite inactivation of fructose-1%2C6-bisphosphatase%3B contains six WD40 repeats%3B computational analysis suggests that Gid7p and Moh1p have similar functions
mutation_affecting_coding_sequence YCL039W_re_remove_BpuEI (462..470) removal of BpuEI
reverse_primer YCL039W_tagr2v1 (299..326)
forward_primer YCL039W_tagf1v1 (659..686)
forward_primer YCL039W_tagf2v1 (80..107)
Sequence (the first 758 bases correspond to coordinates 43217..43974 in synthetic chromosome yeast_chr3_3_23)