BBa_K277066

BBa_K277066 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K277066
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:46

3L.3_23.C1.05



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K277066_sequence (Version 1)

Description

3L.3_23.C1.05 is 719 bases long and is cloned into the pGem-T vector.

3L.3_23.C1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C1.05 is a constituent of 3L.3_23.C1 (along with 3L.3_23.C1.01, 3L.3_23.C1.02, 3L.3_23.C1.03, 3L.3_23.C1.04, 3L.3_23.C1.06, 3L.3_23.C1.07, 3L.3_23.C1.08, and 3L.3_23.C1.09.)

This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
reverse_primer YCL037C_tagr1v1 (492..519)
forward_primer YCL037C_tagf1v1 (249..276)
gene YCL037C (-681..623) Cytoplasmic RNA-binding protein that associates with translating ribosomes%3B involved in heme regulation of Hap1p as a component of the HMC complex%2C also involved in the organization of actin filaments%3B contains a La motif

Sequence (corresponds to coordinates 48531..49249 in synthetic chromosome yeast_chr3_3_23)

Notes

Constructed using overlap extension PCR protocol described in RFC38

Source

Synthetic Yeast

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K277066/1