Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liu Chenli
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:47
sp-eGFP-streptavidin-AIDAc
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K283007_sequence (Version 1) |
Description
Autotransporters are synthesized as pre-proproteins with modular organization. A C-terminal membrane-embedded domain promotes secretion of parts of the protein across the outer membrane and is composed of a β-barrel and a α-helix spanning its lumen. The adhesion involved in diffuse adherence (AIDA-I) is a monemeric autotransporter that has been extensively studied. The cleavage of the autotransporter adhesion involve in diffuse adherence (AIDA-I) of Escherichia coli yields a membrane embedded fragments, AIDAc, and an extracellular fragments, the mature AIDA-I adhesion. In this part, AIDAc directs the fusion protein sp-eGFP-streptavidin-AIDAc (sp means signal sequence) to the outer membrane of the bacteria by the AIDA-I autotransporter pathway. The surface localization of sp-eGFP-streptavidin-AIDAc was demonstrated in our wiki website.In addition, the restriction of autotransporters to the pole is dependent on the presence of a complete lipopolysaccharide (LPS), consistent with known effects of LPS composition on membrane fluidity.Notes
Referenc:Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13)Source
Pathogenic Escherichia coli strain 2787 (O126:H27)The genes encoding AIDA (adhesin involved in diffuse adherence) are located on two open reading frames, orfA and orfB. The latter codes for the AIDA autotransporter system, which is synthesized as a pre-pro-protein that is C-terminally processed to generate the mature adhesin AIDA-I and the outer membrane integrated AIDAC, which functions as the translocator for AIDA-I. orfA codes for a 45-kDa cytoplasmic protein which is required to modify AIDA-I such that it adheres to target cells. Although the AIDA system has first been characterized in a clinical E. coli isolate responsible for infant diarrhea, AIDA genes have been detected only in about 2 to 4% of human isolates . Thus, an animal reservoir for AIDA may exist.