Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nan Hou
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:49
pp1(I type Protein phosphotransferase)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K289001_sequence (Version 1) |
Description
pp1(I type Protein phosphotransferase)is crucial in the MCs detection process.According to the Toxicity mechanism, Adda binding site of MCs has a strong affinity to the lyophobic groove of PP1. Also, according to the Detoxicity mechanism, GSH could construct a covalent bond with Mdha banding site of MCs easily under the function of GST(Glutathione S-Transferase Agents which presents in the cell of Yeast).
In our project, we connect PP1 gene after DNA-BD in the plasmid pGBKT7 and GSH gene after AD in the plasmid pGADT7, and then we transform these two plasmids into Yeast(AH109) so that it can express protein DNA-BD-PP1 and AD-GSH.