BBa_K300996

BBa_K300996 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K300996
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
Date created: 2010-10-01 11:00:00
Date modified: 2015-05-08 01:11:52

SpeI/AvrII mixed site (when SpeI comes from RFC10 Prefix)



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K300996_sequence (Version 1)

Description

SpeI (M31992) and AvrII (BBa_B0048) restriction sites have a compatible overhang (CTAG).

The SpeI-AvrII mixed site is ACTAGG. When SpeI comes from RFC10 BioBrick Suffix, the DNA scar left after a ligation is TACTAGG (see http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix for details).

Notes

Mixed site

Source

-

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K300996/1