BBa_K300997

BBa_K300997 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K300997
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni
Date created: 2010-10-01 11:00:00
Date modified: 2015-05-08 01:11:52

AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix)



    • Details

    Types
    DnaRegion

    Roles
    DNA

    sequence_feature

    Sequences BBa_K300997_sequence (Version 1)

    Description

    AvrII (BBa_B0048) and XbaI (M31991) restriction sites have a compatible overhang (CTAG).

    The AvrII-XbaI mixed site is CCTAGA. When XbaI comes from RFC10 BioBrick Prefix, the DNA scar left after a ligation is CCTAGAG (see http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix for details).

    Notes

    Mixed site

    Source

    Very similar to BBa_B0104, but with an additional "G" nucleotide at 3'.

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K300997/1
     

    Attachments

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