BBa_K305002

BBa_K305002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K305002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dennis Claessen, Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk

Date created: 2010-09-26 11:00:00

Date modified: 2015-05-08 01:11:53

Chaplin C (chpDC) with modified signal sequence and sortase recognition motive

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K305002_sequence (Version 1)

Description

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

Notes

Ordered as construct from Mr. Gene after genomic sequence had been identified and signal sequence + sortase motive had been modified.

Source

codon optimized for B. subtilis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	12,17	ribosome_entry_site, feature/rbs
CDS	25,828	feature/cds, CDS
Signal Sequence	28,117	feature/dna, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K305002/1

Member of these Collections

• iGEM Parts Registry