BBa_K313002

BBa_K313002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K313002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono
Date created: 2010-10-13 11:00:00
Date modified: 2015-05-08 01:11:54

flpe DNA recombinase



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K313002_sequence (Version 1)

    Description

    Flpe is a site-specific DNA recombinase that that recognizes FRT sequence.
    Flpe was derived from yeaset site-specific DNA recombinase Flp.
    (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

    It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.

    Notes

    It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.

    Source

    E.coli EL250 strain

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K313002/1
     

    Attachments

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