BBa_K313002

BBa_K313002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K313002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono

Date created: 2010-10-13 11:00:00

Date modified: 2015-05-08 01:11:54

flpe DNA recombinase

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K313002_sequence (Version 1)

Description

Flpe is a site-specific DNA recombinase that that recognizes FRT sequence.
Flpe was derived from yeaset site-specific DNA recombinase Flp.
(Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.

Notes

It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.

Source

E.coli EL250 strain

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K313002/1

Member of these Collections

• //function/recombination/flp
• iGEM Parts Registry