CapDCP

BBa_K314974 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K314974
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean Wu, Christopher Eiben
Date created: 2010-10-17 11:00:00
Date modified: 2015-05-08 01:11:55

CapDCP_F24Y_L40R_T59N



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K314974_sequence (Version 1)

Description

A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.

Notes

Point mutation at residues 24, 40, 59 on Foldit design from Phenylalanine to Tyrosine, Leucine to Arginine, and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel

Source

In-House Mutant

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K314974/1