Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brianna Pearson
Date created: 2010-06-17 11:00:00
Date modified: 2015-05-08 01:11:55
LoxBri (R)variant reverse lox site with 3 base changes in the spacer region
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K315006_sequence (Version 1) |
Description
The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If the one site is forward and the other reverse, the coding sequence will be inverted. This variant will not recombine with other lox sites because of the mutations in the spacer region.Notes
In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.Source
We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735).[edit]
| Sequence Annotation | Location | Component / Role(s) |
| inverted repeat spacer region inverted repeat C -> T C -> G A -> T | 1,13 14,21 22,34 15,15 19,19 20,20 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/mutation sequence_alteration sequence_alteration feature/mutation feature/mutation sequence_alteration |