BBa_K315018

BBa_K315018 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K315018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brianna Pearson

Date created: 2010-07-08 11:00:00

Date modified: 2016-02-10 02:50:27

pTet-loxP-RBS-RFP-loxBri(F)

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K315018_sequence (Version 1)

Description

This is a device that will test the recombination activity of Cre using a floxed RFP protein and variant lox sites. The cells should still glow red in the presence of Cre because the lox sites are not compatible.

Notes

I used a moderate promoter and a oligos I designed with three mutations in order to test the interaction between variant lox sites.

Source

GCAT-alog.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	engineered_region, feature/BioBrick
TetR 1	1,19	feature/binding, non_covalent_binding_site
-35	20,25	promoter, feature/promoter
TetR 2	26,44	feature/binding, non_covalent_binding_site
-10	43,48	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K315018/1

Member of these Collections

• iGEM Parts Registry