BBa_K315023

BBa_K315023 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K315023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brianna Pearson

Date created: 2010-07-14 11:00:00

Date modified: 2016-02-10 02:43:15

pTet-loxBri(F)-RBS-RFP-loxBri(F)

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K315023_sequence (Version 1)

Description

This device shows the activity of Cre on variant lox sites with three point mutations in the inverted arm region. If these arms are compatible, as they should be, then RFP will be excised and cells will no longer glow red in the presence of Cre.

Notes

pTet is a moderate promter and floxed RFP is an immediate indicator of excision.

Source

GCAT-alog of Davidson

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	feature/BioBrick, engineered_region
TetR 1	1,19	non_covalent_binding_site, feature/binding
-35	20,25	feature/promoter, promoter
TetR 2	26,44	non_covalent_binding_site, feature/binding
-10	43,48	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K315023/1

Member of these Collections

• iGEM Parts Registry