Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danielle Wagner
Date created: 2010-07-20 11:00:00
Date modified: 2015-05-08 01:11:56
pLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K315031_sequence (Version 1) |
Description
This sequence contains the wild type TetA gene which has been optimized from base pairs 1 to 290. This composite part has the construct of pLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is optimized, we are hypothesizing that it will allow for greater translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis). This TetA gene has been optimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.Notes
During the design of this sequence, it was necessary to ensure that the optimized sequence did not contain BioBrick restriction sites and that the translational sequence was identical to that of the wild type gene.Source
This TetA gene has been optimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was constructed using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.| Access | Instance | Definition |
| public public | BBa_S04446 BBa_K315040 | BBa_S04446 BBa_K315040 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_S04446 BBa_K315040 | 1,952 959,2148 | BBa_S04446 BBa_K315040 |