Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Curtiss Lane
Date created: 2010-07-21 11:00:00
Date modified: 2015-05-08 01:11:56
pLacI-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K315044_sequence (Version 1) |
Description
This sequence contains the wild type TetA gene which has been deoptimized from base pairs 1 to 290. This composite part has the construct of pLacI-RBS-RFP-RBS-TetA (deoptimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).Notes
This TetA gene has been deoptimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.Source
This TetA gene has been deoptimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.| Access | Instance | Definition |
| public public | BBa_S04447 BBa_K315041 | BBa_S04447 BBa_K315041 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_S04447 BBa_K315041 | 1,808 815,2005 | BBa_S04447 BBa_K315041 |