Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:11:56
XylE - catechol 2,3-dioxygenase from P.putida with terminator
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K316003_sequence (Version 1) |
Description
Catechol or catechol 2,3-dioxygenases + O(2) is converted by a ring cleavage into 2-hydroxymuconate semialdehyde which is the toxic and bright yellow-coloured substrate. This is a key enzyme in many (soil) bacterial species used for the degradation of aromatic compounds. The enzyme used here itself originating from Pseudomonas putida is a homotetramer of C230 monomers. The tetramerization interactions position a ferrous ion critical for enzymatic activity. It has been deduced that intersubunit interaction is essential to produce a functioning enzyme after performing N and C terminal modifications on the monomer. Coming together the subunits generate an active site. The reaction itself takes place within seconds after the addition by Pasteur pipette or spraying of catechol at a 100mM stock solution diluted with DDH20 (used by our lab.) The toxic byproduct is thought to interfere with cell wall integrity and cellular machinery such that exposed cells gradually die.Notes
The parts were put together using standard assembly http://partsregistry.org/Assembly:Standard_assemblySource
XylE was obtained from the registry| Access | Instance | Definition |
| public public | BBa_J33204 BBa_B0014 | BBa_J33204 BBa_B0014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33204 BBa_B0014 | 1,958 967,1061 | BBa_J33204 BBa_B0014 |