BBa_K316007

BBa_K316007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K316007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: IC 2010 Team

Date created: 2010-10-20 11:00:00

Date modified: 2015-05-08 01:11:56

Recombinant GFP-XylE protein under Pveg promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K316007_sequence (Version 1)

Description

This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays.

Notes

Standard biobrick cloning of intermediary parts

Source

Pveg and spoVG biobricks BBa_K143053 added to 5'his tagged-GFP-XylE construct BBa_K316004.

Components

Access	Instance	Definition
public	BBa_K143053	Pveg-spoVG
public	BBa_K316006	GFP-XylE

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143053	1,117	Pveg-spoVG
BBa_K316006	124,1845	GFP-XylE

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K316007/1

Member of these Collections

• iGEM Parts Registry