ComE

BBa_K316016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K316016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:11:56

ComE transcription factor phosphorylation-activated by ComD



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K316016_sequence (Version 1)

Description

ComE is the response regulator of a two-component signal transduction signaling pathway in a Streptococcus pneumoniae quorum sensing system. It is phosphorylate and activated by ligand-bound ComD. It will then induce transcription of any gene which has a ComE binding site in its promoter

Notes

Synthetic RBS to optimise translation in B. subtilis as part of poly-mRNA containing ComD upstream

Source

Streptococcus pneumoniae, AAC44897, codon optimised for expression in B. subtilis

Sequence Annotation Location Component / Role(s)
pVEG
Synthetic RBS
ComE Start Codon
ComE
Stop Codon
Stop Codon
1,97
106,117
124,126
124,897
901,903
898,900
promoter feature/promoter
feature/rbs ribosome_entry_site
start_codon feature/start
CDS feature/cds
stop_codon feature/stop
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K316016/1