Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:11:56
LytC cell wall anchoring protein for B. subtilis
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K316030_sequence (Version 1) |
Description
The cell wall binding domin of lytC, a N-acetylmuramoyl-L-alanine amidase of B. subtilis, was isolated to be used as a cell wall anchor. As demonstrated by Kobayashi et al. 2000 this domin can be used to target catalytic domians of other proteins as well as peptides to the cell wall of B. subtilis whilst leaving their functionality intact."Notes
Pfu DNA polymerase was used to minimise PCR errorsSource
Genome PCR from B. subtilis genome (strain)| Sequence Annotation | Location | Component / Role(s) |
| Synthetic RBS coding scar LytC 2-953 of 1491 | 1,15 16,21 122,974 | ribosome_entry_site feature/rbs feature/misc sequence_feature CDS feature/protein |